mCherry mRNA (5moU)

This RNA encodes mCherry, a protein that can produce a red fluorescent signal when expressed in cells. This makes it a useful tool for studying drug delivery, gene expression, protein localization, and other biological processes.

mCherry is a fluorescent RNA molecule that can be used for live-cell imaging and gene expression studies. It is derived from the mCherry protein, which is a widely used tool in molecular biology for labeling and tracking cells and proteins.

Description

Live-cell imaging of gene expression

• mCherry RNA can be used to visualize and image transcription and expression in live cells. This technique is useful for studying drug delivery uptake and potency, particularly when sample auto–fluorescence interferes with commonly used “green” reporters.

Screening and optimization with flow cytometry

• Flow based analytical methods provide quantitative data for drug delivery system evaluation and optimization.

Screening of gene editing and CRISPR-Cas9 systems

• mCherry RNA can also be used to screen for successful gene editing events mediated by CRISPR-Cas9 or other gene editing systems. By co-transfecting mCherry RNA with the gene editing machinery (i.e. Cas9 RNA) and monitoring the appearance of fluorescent cells, you can easily identify cells that have undergone gene editing and select them for further analysis.