LNP Formulation Calculator

Easily calculate component ratios and quantities for your lipid nanoparticle formulations.

Step 1: Define Lipid Composition

Ionizable Lipid:

g/mol

#N per

Lipid

mol%

Helper Lipid:

g/mol

Cholesterol:

g/mol

PEG-Lipid:

g/mol

Step 2: Define Target mRNA Inputs

Start by entering the amount of mRNA you are working with:

Mass of RNA To Encapsulate

Buffered RNA Solution Concentration

ug/mL

Buffered RNA Volume

Please provide Input RNA Mass, and the Target Buffered RNA Concentration or Volume.

Step 3: Input Flow Rate Ratio (FRR) or Mixing Ratio

FRR (aqueous/RNA : organic)

Final Sample RNA Concentration

ug/mL

Final Sample Volume

Required Lipid Master Mix Volume

Update the Flow Rate Ratio (FRR) value to see the effect on Final RNA Sample Concentration, Total Final Sample Volume, and required Lipid Master Mix Volume/Concentration

Step 4: Select N/P or w/w Ratio

Select method for Lipid-to-RNA Ratio

Required Lipid Master Mix Conc.

Update N/P or w/w ratio to see real-time effects on required Lipid Master Mix Total Concentration

Final RNA Concentration (Check)

ug/mL

Final Lipid Concentration (Check)

Waiting for inputs...

Lipid Master Mix Preparation

Calculate the volumes of individual lipid stock solutions to be combined when creating a custom lipid master mix in organic solvent, based on your target total concentration and molar ratios.

NOTE: See Step 1 to change lipids, desired composition, or enter custom lipids and molecular weight values.

Desired Total Lipid Master Mix Concentration

Desired Total Lipid Master Mix Volume

Calculated Formulation Parameter Details

1. Ionizable Lipid Stock Concentration

2. Helper Lipid Stock Concentration

3. Cholesterol Stock Concentration

4. PEG Lipid Stock Concentration

Volume of Additional Solvent (Q.S) to reach Volume

Final Volume

Waiting for inputs...

Single Lipid Stock Conversion

Select Lipid

MW (g/mol)

Concentration (mM)

Concentration (mg/mL)

Single Lipid Stock Preparation

Enter a mass and determine the volume of solvent to add, or update the target volume to see how much lipid mass is required.

Lipid mass (mg)

Volume (mL)

✅ Inputs valid. Conversion ready.

Buffered RNA Solution Preparation

Calculate the volumes of RNA stock solution, buffer, and water to be combined when diluting RNA for nanoparticle synthesis, based on your target total RNA concentration/volume and starting material concentrations.

Target Buffered RNA Solution Concentration

Target Buffered RNA Solution Volume

Buffer Details

Buffer Type

Target Final Buffer Concentration

RNA Dilution and Buffer Mixing Details

1. Starting RNA Stock Solution

2. Starting Buffer Stock Solution

Volume of Additional Water (Q.S) to reach Volume

Final Volume

✅ Mix 200.0 uL RNA stock, 33.3 uL Sodium Acetate stock, and 766.7 uL water → 1.000 mL buffered RNA at 200 ug/mL + 100 mM Sodium Acetate

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Contact

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LNP Formulation Calculator

Step 1: Define Lipid Composition

Step 2: Define Target mRNA Inputs

Step 3: Input Flow Rate Ratio (FRR) or Mixing Ratio

Step 4: Select N/P or w/w Ratio

Step 2: Define Input mRNA and Lipid Stock Concentrations

Step 3: Input Flow Rate Ratio (FRR) or Mixing Ratio

Calculated N to P Ratio

Lipid Master Mix Preparation

Calculated Formulation Parameter Details

Single Lipid Stock Conversion

Single Lipid Stock Preparation

Buffered RNA Solution Preparation

Buffer Details

RNA Dilution and Buffer Mixing Details

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