BrightQuant™
Nucleic Acid Quantification Assay
BrightQuant™ is a fluorescence-based reagent and kit platform for quantifying nucleic acid concentration, including RNA, DNA, and oligonucleotides, both inside and outside nanoparticles.
Specially formulated for complex nanoparticle samples, BrightQuant™ is optimized for calculating EE % in formulations containing ionizable or cationic lipids, proteins, and polymer-based nanoparticles.
BrightQuant™
Two Streamlined Pathways for RNA Quantification
Encapsulated RNA / Nanoparticle EE% Analysis
BrightQuant™ R Nanoparticle Encapsulation Efficiency (EE%) Kit is designed for determining free RNA, total RNA, encapsulated RNA, and encapsulation efficiency in lipid nanoparticles (LNPs) and related nanoparticle formulations.
Contains:
BrightQuant™ R Reagent
20X TE Buffer
20X BrightQuant™ Omni NP Digest Buffer
mRNA Standard, 1kb
BrightQuant™ for Complex Nanoparticle Formulations
Omni NP Digest Buffer
Helix Biotech's digestion buffer blend designed to disrupt nanoparticle structures that can otherwise mask encapsulated nucleic acids during analysis. Ideal for calculating LNP encapsulation efficiency.
-
Works across complex formulations.
Supports cationic and ionizable lipid nanoparticles as well as select polymer and protein-based systems.
-
Releases enclosed payloads.
Efficient digestion enables downstream fluorescence-based quantification of RNA, DNA or oligonucleotides.
Streamlined Workflow to Determine Nanoparticle Encapsulation Efficiency %
RNA Quantification.
Reliable Results.
Compare BrightQuant™ with Ribogreen™ across RNA quantification and nanoparticle encapsulation efficiency measurements.
Signal (485/20, 528/20)
*RiboGreen™ is a registered trademark of Thermo Fisher Scientific. BrightQuant™ R is not affiliated with or endorsed by Thermo Fisher Scientific.”
FAQ
What is BrightQuant™ used for?
BrightQuant™ is used for nucleic acid quantification, helping users measure RNA or other nucleic acids with a fluorescence-based assay.
What is Omni NP Digest Buffer for?
Omni-NP Digest Buffer is designed to disrupt cationic and ionizable lipid, polymer, and protein-based nanoparticles so encapsulated nucleic acids can be released for quantification.
How is BrightQuant™ similar to RiboGreen™-style assays?
BrightQuant™ works like RiboGreen™-style assays in that it uses a fluorescence signal and a standard curve to quantify nucleic acids, but it is designed for a broader range of sample types. It performs just as efficiently for routine RNA extracts while also being optimized for complex formulations such as lipid nanoparticles, liposomes, polymer nanoparticles, and other blended delivery systems.
Do users switching from RiboGreen™ need to change how they think about the workflow?
No! Users already familiar with fluorescence-based RNA quantification, standard curves, and plate or fluorometer readouts should find the BrightQuant workflow conceptually familiar.
What read settings are typically used for BrightQuant™ workflows?
BrightQuant™ assays are typically read at approximately 485 nm excitation and 520–530 nm emission, depending on the instrument and assay configuration. For initial runs, gain should be optimized, and auto-gain set to the highest standard wells can help establish consistent RFU values, linearity, and accuracy.
What is encapsulation efficiency?
Encapsulation efficiency is the percentage of total nucleic acid that is successfully enclosed inside nanoparticles, calculated by comparing encapsulated RNA to total RNA.
