BrightQuant™ R EE enables rapid, accurate determination of RNA nanoparticle encapsulation efficiency (EE%) using standard fluorescence plate readers and common fluorescence filter sets. The kit includes BrightQuant™ R reagent, RNase-free TE buffer, BrightQuant™ Omni NP Digest Buffer, and RNA calibration standards for quantifying free RNA, total RNA, encapsulated RNA, and encapsulation efficiency in lipid nanoparticles (LNPs) and other nanoparticle formulations.

Kit Contents

• 1 mL BrightQuant™ R Reagent (RNA)

• 25 mL 20X TE Buffer

• 25 mL 20X BrightQuant™ Omni NP Digest Buffer

• 5 × 100 µL BrightQuant™ mRNA Standard (1 kb)

Applications

• Free RNA quantification

• Total RNA quantification

• Encapsulation efficiency (EE%)

• Lipid nanoparticles (LNPs)

• Liposomes

• Polymeric nanoparticles