FLuc mRNA (5moU)

This RNA encodes Firefly Luciferase which is an enzyme that produces bioluminescence, when combined with a molecule called luciferin. This bioluminescent signal can be measured using specialized instruments and can be used to track gene expression and protein activity in living cells and animals.

 

Scientists can use this tool to study how genes are regulated in response to different stimuli or conditions, such as disease or environmental changes. By introducing the RNA that encodes for Firefly Luciferase into cells or animals, they can track the expression and activity of specific genes or proteins over time. This information can help researchers better understand how biological processes work and identify potential targets for drug development.

 

Description

Product Name	Firefly Luciferase (FLuc) mRNA
Length	1939 nt
Modifications	5-Methoxyuridine (5moU)
Appearance	Clear, Colorless Solution
Buffer	1mM Sodium Citrate, pH 6.4
Purity	>90%, Fragment analysis by capillary gel electrophoresis
Concentration	~1 mg/mL
A260/280	~2.0
Endotoxin	<10 EU/mg
Storage	≤ -40°C
Shipping Method	Dry Ice

 

Drug delivery studies

• Fluc RNA is commonly used as a reporter to study transfection potency, cellular uptake, and localization with nanoscale drug delivery formulations.

Screening and optimization with flow cytometry

• Flow based analytical methods provide quantitative data for drug delivery system evaluation and optimization.

Biodistribution, In Vivo

• Fluc RNA is used with in vitro imaging systems to investigate biodistribution with LNPs and other nanocarriers.