When Should You Use a Standard LNP Composition versus a Custom Lipid/Polymer Formulation?

Moving a lipid- or polymer-based nanoparticle formulation from lab scale to GMP manufacturing is a complex process. Tech transfer is a critical stage where many projects face delays, increased costs, or regulatory hurdles due to missing or unclear steps. Careful preparation before engaging a Contract Development and Manufacturing Organization (CDMO) is essential to avoid these pitfalls. By approaching the transfer methodically, you can significantly reduce risk and ensure you

Most mRNA programs begin the same way. A coding sequence or gene of interest is selected. A codon optimization tool is run. The GC content looks acceptable. The sequence is ordered. Then expression is lower than expected, stability is inconsistent, innate immune activation appears, and manufacturing yields are unpredictable. The assumption was simple: optimize codon frequency and translation will improve. Reality is more complex. Codon optimization is only one variable in a m

This article compares turbulent mixers (solvent injection) and extrusion methods, highlighting their advantages, limitations, and practical applications to help you decide which method suits your needs best.

In recent conversations with clients, there's been a surge of interest around a rather intriguing subject in the biotech world: lipid nanoparticles (LNPs) and, specifically, the state-of-the-art mixing techniques used in their production. Clients do not know the difference between the turbulent mixing approach and that of microfluidics - they often come to us to enlighten them and explain which one is better! Over the years, our team has witnessed the evolution of numerous m

Nine elephant calves at the Houston, Fort Worth, Rosamond Gifford, and Cincinnati zoos, received a groundbreaking EEHV mRNA-LNP vaccine developed through the efforts between Baylor College of Medicine, Helix Biotech, Avanti Polar Lipids/Croda and partner zoos.

Lipid nanoparticles (LNPs) have become essential tools in delivering nucleic acids like mRNA and siRNA for vaccines and gene therapies. A critical but often overlooked aspect of LNP development is the choice of formulation buffers. These buffers influence particle stability, encapsulation efficiency, and ultimately, clinical success. This guide breaks down the common buffers used in LNP formulation and storage, highlights what commercial and clinical products use, and helps y

RNA therapeutics are transforming the future of healthcare with targeted gene therapies. What are RNA therapeutics?  RNA molecules,...

The development of liposomal drug formulations has transformed drug delivery by improving therapeutic efficacy, reducing toxicity, and...

Liposomes  are a leading technology in targeted drug delivery systems, playing an essential role in transforming how treatments reach...

Over the past decade, gene therapy has made incredible strides, offering potential cures for a wide range of genetic disorders, cancers,...

Gene therapy has emerged as a transformative approach for treating various genetic disorders, cancer, and rare diseases, offering the...

Helix Biotech's Expertise Recognized in Recent JoVE Publication Figure 1 - JoVE article. At Helix Biotech, we are proud to announce our...

PEG lipids gained worldwide prominence during the SARS-CoV-2 global pandemic.

Lipid nanoparticles (LNPs) have played a groundbreaking role in the area of vaccines.

Focusing on strategies to optimize drug loading and ensure the successful delivery and translation of mRNA.

Introduction The delivery of genetic material holds profound implications for therapeutic treatment of a variety of diseases and...

Lipid nanoparticles (LNPs) have emerged as a pivotal player, revolutionizing the landscape of drug development. These minuscule yet...

From blending spirits for the most exquisite cocktails to intricately mixing lipids and nucleic acids for life-saving lipid nanoparticles...