Helix Biotech's EZNano LP-01 LNP is a pre-formulated lipid nanoparticle with the following components:
45% - LP-01 (Ionizable Cationic Amino Lipid)
9% - 1,2-distearoyl-sn-glycero-3-PC (1,2-DSPC, Phospholipid)
44% - Cholesterol
2% - DMG-PEG(2000) (Lipid Excipient)
The EZNano LP-01 LNP can come with a variety of lipophilic dyes (DiO, DiI, DiD, and DiR), and can be loaded with mCherry, eGFP or FLuc mRNA.
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EZNano LP-01 LNP
*Empty LNPs are provided at an equivalent concentration of lipids and volume.
**EZNano LNPs include a small amount of cryoprotectant to aid in longterm frozen storage.
Table 1. mRNA-LNP QC
Attributes Specifications Particle Size 70-100 nm PDI <0.2 Encapsulation Efficiency >90% mRNA Concentration 100 µg/mL ± 10% Dye Concentration 0.1%, mol Volumes 5.5µg = 55µL or 11µg = 110µL Storage -80°C *QC data measured after subjecting LNPs to freeze-thaw cycle
Table 2. Excitation & Emission Values
Dye/Fluorescent Protein Excitation (nm) Emission (nm) eGFP 488 509 Fluc (Firefly Luciferase) - 560 mCherry 587 610 DiO 484 501 DiI 549 565 DiD 644 665 DiR 750 780